Job Description

Technician

Berkeley, CA

6 Month Initial Contact

Pay: $25-28/hr

SCHEDULE OPTIONS:

• 6am to 2pm
• 6:30am to 3pm
• 2:30pm to 11pm
• 6pm to 2am
• 10:30pm to 7am

*No shift differential in pay

Description:

• Cell Culture bioreactor system assembly and use, including control of gas supply to bioreactor, Micro-sparger assembly, Anti-Foam Emulsion system. Ability to troubleshoot and control processes.
• Continuous Cells Separation equipment preparation and process/controls.
• Membrane Absorption Chromatography process (e.g. Membrane Absorption process, Buffer and NaOH supply, cTCF supply, Capsule preparation, Inline dilution and Temperature controls).
• Basic knowledge of protein chemistry and purification systems such as anion, cation, and immunoaffinity chromatography.
• Membrane Absorber technology, viral removal techniques and tangential flow filtration technology. Large scale chromatography, TFF technology.
• Familiarity with cGMP’s and applicable agency regulations
• Prepares process equipment (including but not limited to Bioreactors & ancillary equipment,
• Stainless Steel tanks, Pressure Transmitters, Flow Meters, Aggregate traps, Harvest discards, Transfer lines (buffers, cTCF), Hoses and small parts.
• Cleans and Sterilizes equipment using appropriate CIP (Clean-In-Place), SIP (Steam-In?Place) procedures.
• Performs daily or routine process operations in the cell culture and purification of biotech and biological products (Kovaltry, Kogenate, and Jivi). Operates equipment for these processes including:
• Inoculates and monitors bioreactors.
• Processes cTCF material to Continuous Cell Separation (CCS).
• Operates Chromatography, MAEF, MAVF and TFF skids.
• Processes MAEF material using closed thaw process, step elution chromatography, Membrane Absorber and filter clearance technology, and gradient controlled SP column separation techniques.
• Operates 50L, 200L and 1000L collection bag technology to ensure robust collection, mixing and dilution of products.
• Performs PEGylation of JIVI in process material using TFF technology.
• Operates automated bagging systems.
• Blast Freezes product for storage.
• Dispenses chemicals and formulate solutions.
• Prepares acids and bases as necessary.
• Performs aseptic operations.
• Takes, analyzes and submits samples as required throughout the process.
• Monitors and controls operation with computer control systems.
• Completes all routine check sheets.
• Cleans equipment and maintains clean and orderly production facilities.
• Performs in-depth troubleshooting and root cause analysis of Kovaltry, Kogenate, and Jivi equipment and process failures.
• Contributes to continuous improvement opportunities. Performs all operations in compliance with cGMP.
• Performs other related duties as assigned.

Required Qualifications:

• Requires completion of two-years’ college science courses in (e.g. biology, chemistry, biochemistry, or microbiology) or a certificate from an accredited bio-technology academy.

• Relevant bio-tech industry experience in some or all of the following is preferred, and may be considered in lieu of some or all of the educational requirements above

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